Reconstitution calculator.
Lyophilised material arrives as a dry powder, and the volume of solvent you add is what sets its concentration. This works out that concentration, and the volume that holds a given amount — as millilitres, and as units on a U-100 syringe.
The arithmetic is the same whichever compound is in the vial. Read it alongside reconstitution and storage basics, which covers the handling this page assumes.
The mass printed on the vial — or the measured figure on its certificate.
You choose this. It sets the concentration and nothing else.
A unit is a volume, not an amount.
The numbers on an insulin syringe are graduations of volume — 100 units is one millilitre, whatever is dissolved in it. The same vial reconstituted with 1 mL and with 3 mL gives readings three times apart for an identical mass of peptide. That is why the solvent volume is an input here and not an assumption, and why a figure copied from someone else's protocol means nothing without theirs.
Educational only, and not medical advice. Research-use materials are not for human or animal consumption. Concentration figures are only as good as the mass they start from — use the measured value on the lot's certificate rather than the number printed on the label where the two differ.